Optimized Protocol of Methanol Treatment for Immunofluorescent Staining in Fixed Brain Slices
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چکیده
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Optimized Protocol of Methanol Treatment for Immunofluorescent Staining in Fixed Brain Slices
We optimized methanol treatment in paraformaldehyde-fixed slices for immunofluorescent staining of ependymal basal bodies in brain ventricles. As 100% methanol induced severe deformations to the slices (including rolling and folding over), we tried to decrease methanol concentration. We found that 33.3% to 75% methanol could result in ideal immunostaining of basal bodies without inducing obviou...
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ژورنال
عنوان ژورنال: Applied Immunohistochemistry & Molecular Morphology
سال: 2017
ISSN: 1541-2016
DOI: 10.1097/pai.0000000000000293